| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110114 |
| Chromosome: | chromosome 8 |
| Location: | 4058666 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGCACCCGTTCCCATGCACAGCGTGCC |
| Internal bar code: | CAGCCGGCGGGATCAAACCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 98.7453 |
| LEAP-Seq n confirming: | 4722 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACACACAATCGCATCAT |
| Suggested primer 2: | AGCAGCTGGTGTTGTGTCTG |