| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110115 |
| Chromosome: | chromosome 9 |
| Location: | 180314 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386740 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGCCCACTGGTTTTTCATCTCCCAGG |
| Internal bar code: | GCTGAGTCGAGACAGCCCACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 167 |
| LEAP-Seq percent confirming: | 98.693 |
| LEAP-Seq n confirming: | 3398 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGCCTTGGCTGTCTCAC |
| Suggested primer 2: | CCGAGCACTTTATGCTGTGA |