| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110115 |
| Chromosome: | chromosome 12 |
| Location: | 4643902 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523026 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATCCTATCCCACAGCAGGAGGCAGGCA |
| Internal bar code: | TTCGAGTATCCGATGGGAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 810 |
| LEAP-Seq percent confirming: | 98.8987 |
| LEAP-Seq n confirming: | 9519 |
| LEAP-Seq n nonconfirming: | 106 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCAAGGTCTCAGTGAGTG |
| Suggested primer 2: | TGTCACATCCGACTCCACAT |