| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110122 |
| Chromosome: | chromosome 6 |
| Location: | 2961978 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273150 | RRM4 | (1 of 2) 2.1.1.166 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase / Um2552 methyltransferase; Putative ribosomal RNA methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGCGCTGCCGGTCCCCGTACGGGAGGT |
| Internal bar code: | AGAACGGCACGGTGACGTGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 62.1118 |
| LEAP-Seq n confirming: | 3000 |
| LEAP-Seq n nonconfirming: | 1830 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGTGAAGAGTGATGTGG |
| Suggested primer 2: | AGAGCTGCCAACCGTTCTTA |