| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110124 |
| Chromosome: | chromosome 3 |
| Location: | 631144 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145747 | SHK7,SHKH1 | (1 of 1) 4.2.3.5 - Chorismate synthase / 5-enolpyruvylshikimate-3-phosphate phospholyase; Chorismate synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATGATTGACGGTGGGTGTCGGCTGGGAG |
| Internal bar code: | CACCGCTCCTAGGCCGTGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 113 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 165 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGCTTTCATTTTATGGGC |
| Suggested primer 2: | CTCAAAGCCCTGAAGCGTAG |