| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110128 |
| Chromosome: | chromosome 6 |
| Location: | 3520046 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278108 | (1 of 5) PF13508 - Acetyltransferase (GNAT) domain (Acetyltransf_7) | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACAGGGGGCCTTCCGTTCAGGGGAACG |
| Internal bar code: | ATGCAGACGCGGTCGACTATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 98.5567 |
| LEAP-Seq n confirming: | 478 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGTCGGTCTTCACCTT |
| Suggested primer 2: | TTTACAGACTCGCAGCATGG |