| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.110136 |
| Chromosome: | chromosome 3 |
| Location: | 7448224 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205750 | (1 of 2) K18272 - protein XRP2 (RP2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGAACACTGCGCCGTCGGTGGGACCTG |
| Internal bar code: | GGGCGCTGGTTCACTTCGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1345 |
| LEAP-Seq percent confirming: | 99.5791 |
| LEAP-Seq n confirming: | 8517 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTCTACTGAGGCCCTGC |
| Suggested primer 2: | GCATACCTGCTAGCTGAGGG |