| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.110138 |
| Chromosome: | chromosome 6 |
| Location: | 2353675 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267550 | BBS5 | Bardet-Biedl syndrome-5 associated protein; (1 of 1) K16748 - Bardet-Biedl syndrome 5 protein (BBS5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGAAGGCGCGCGTGCGTGTATGGTCAT |
| Internal bar code: | TTTTATGCTTATTGTGCTTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 678 |
| LEAP-Seq percent confirming: | 99.6198 |
| LEAP-Seq n confirming: | 13101 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCATAGCGTAGCATCTGT |
| Suggested primer 2: | GCCCTGTGCGTGTGTATATG |