Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.110139 |
Chromosome: | chromosome 4 |
Location: | 1335878 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g214650 | BGS5,GSL1,GTR19 | Glucan synthase-like 1; (1 of 7) 2.4.1.34 - 1,3-beta-glucan synthase / UDP-glucose-1,3-beta-D-glucan glucosyltransferase | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACAATCGGAGACTCTCGCCTGATGATGG |
Internal bar code: | GTACCACCTTAGGGGCGTGCTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 416 |
LEAP-Seq percent confirming: | 99.3904 |
LEAP-Seq n confirming: | 3587 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TAGCTGGATGTCTGCTGGTG |
Suggested primer 2: | GCGTGCTCTATTCATCCCTC |