Insertion junction: LMJ.RY0402.110139_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.110139
Chromosome:chromosome 4
Location:1335878
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g214650 BGS5,GSL1,GTR19 Glucan synthase-like 1; (1 of 7) 2.4.1.34 - 1,3-beta-glucan synthase / UDP-glucose-1,3-beta-D-glucan glucosyltransferase gene_edge/mRNA_edge/3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACAATCGGAGACTCTCGCCTGATGATGG
Internal bar code:GTACCACCTTAGGGGCGTGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:416
LEAP-Seq percent confirming:99.3904
LEAP-Seq n confirming:3587
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCTGGATGTCTGCTGGTG
Suggested primer 2:GCGTGCTCTATTCATCCCTC