| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110139 |
| Chromosome: | chromosome 10 |
| Location: | 4093329 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449700 | (1 of 7) PF00582 - Universal stress protein family (Usp) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAACCAGCAGACCAGGAGGGGCCGGGAA |
| Internal bar code: | TCTAGATGCATCGAACTGTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 99.2138 |
| LEAP-Seq n confirming: | 631 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCCACATTGTAGACGTG |
| Suggested primer 2: | GAAGTCCGCATATTGCAGGT |