Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.110148 |
Chromosome: | chromosome_14 |
Location: | 736255 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g612700 | DRC7,FAP50 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCCAGGCAAAAGGAAACGTCGTTGGGACAC |
Internal bar code: | ATGGTCCTCCAGACTATTATCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 481 |
LEAP-Seq percent confirming: | 99.6073 |
LEAP-Seq n confirming: | 1522 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCACCATTCAGCCTAAGGAA |
Suggested primer 2: | GGTGCATGTGATTCTTGGTG |