Insertion junction: LMJ.RY0402.110167_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.110167
Chromosome:chromosome 15
Location:573971
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre15.g637282 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATAGGAACAAACTACCCCCCCCCTGTCCCT
Internal bar code:ATCCAGTGTTCGGGACGTGCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:250
LEAP-Seq percent confirming:98.9337
LEAP-Seq n confirming:1299
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTGGTGTTGTTGTTGCT
Suggested primer 2:GGCATATCACTCCCCTCTCA