| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110169 |
| Chromosome: | chromosome 16 |
| Location: | 5036160 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684550 | HAR2 | Possible 3-hydroxyacid dehydrogenase; (1 of 3) 1.1.1.26 - Glyoxylate reductase / NADH-dependent glyoxylate reductase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGCAGGTCCCAAGGAATCGGCAGCCCT |
| Internal bar code: | CCCGGGGCATAGCAAAGAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 681 |
| LEAP-Seq percent confirming: | 98.8722 |
| LEAP-Seq n confirming: | 263 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTGATGTCGCTGGCTAA |
| Suggested primer 2: | TTGTTTGCAAGAAATGCTGC |