Insertion junction: LMJ.RY0402.110170_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.110170
Chromosome:chromosome 3
Location:6199504
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g192350 (1 of 2) IPR006652//IPR015915 - Kelch repeat type 1 // Kelch-type beta propeller 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGACGTGTCTGTACGGCGTTTACCTTCT
Internal bar code:CATCAATTTCTGGTACAAGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:333
LEAP-Seq percent confirming:93.0969
LEAP-Seq n confirming:1470
LEAP-Seq n nonconfirming:109
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCGACAACACGTCAATCAAC
Suggested primer 2:ACAAACGCAGCGAGTTCTTT