| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110170 |
| Chromosome: | chromosome 3 |
| Location: | 6199507 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192350 | (1 of 2) IPR006652//IPR015915 - Kelch repeat type 1 // Kelch-type beta propeller | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTATCAAACGCACACACCTACACACATCG |
| Internal bar code: | ACTACTTCGGGGGGTGATGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 893 |
| LEAP-Seq percent confirming: | 99.8261 |
| LEAP-Seq n confirming: | 1148 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGACAACACGTCAATCAAC |
| Suggested primer 2: | ACAAACGCAGCGAGTTCTTT |