Insertion junction: LMJ.RY0402.110178_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.110178
Chromosome:chromosome 6
Location:1748411
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g261950 ANK11 (1 of 29) PF13637 - Ankyrin repeats (many copies) (Ank_4); Predicted protein with ankyrin repeats 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGGGATGGGTGTGCCGTGGTGCTGCGGG
Internal bar code:GGAGTCAGGAGCAGGGGTCCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:331
LEAP-Seq percent confirming:99.3939
LEAP-Seq n confirming:164
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GACGAGGGACTGAAAAACCA
Suggested primer 2:AGGTGTAATGTCGCCTCACC