| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.110186 |
| Chromosome: | chromosome 7 |
| Location: | 3345462 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334900 | (1 of 3) IPR004089 - Methyl-accepting chemotaxis protein (MCP) signalling domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCATCAGGACCAGCCCGCACACTTCTC |
| Internal bar code: | AATCTGCCGTGGTCTTTCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 746 |
| LEAP-Seq percent confirming: | 99.4831 |
| LEAP-Seq n confirming: | 4234 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGAGGCGGACAAGGTAA |
| Suggested primer 2: | GCCTGACCCTGTACCCTGTA |