Insertion junction: LMJ.RY0402.110191_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.110191
Chromosome:chromosome 2
Location:9144362
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g143467 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGTCCCATCACGCACCACACAACACCAC
Internal bar code:CGTCGGGATCCCCCTTGGAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:748
LEAP-Seq percent confirming:32.9897
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:65
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GAATCTCGCACCACACAATG
Suggested primer 2:ACCAAACCACAGTGAGGAGG