| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110196 |
| Chromosome: | chromosome 1 |
| Location: | 2676245 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015750 | (1 of 2) PF07978 - NIPSNAP (NIPSNAP) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGGGCGAAGCTGACCTAGGATCTGGGA |
| Internal bar code: | GTTCTCGTGGATTCTCTAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 99.7396 |
| LEAP-Seq n confirming: | 1149 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTTTCGGTCCCTTCTGCT |
| Suggested primer 2: | GCAGGGTTGTCATACTCCGT |