Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.110202 |
Chromosome: | chromosome 2 |
Location: | 6451072 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g116000 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGACCAGCATACACTGGTAGCATTTGC |
Internal bar code: | AAGCGCGGTCGGTCAAGAACTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 476 |
LEAP-Seq percent confirming: | 99.7118 |
LEAP-Seq n confirming: | 346 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTGCCTCCGAATAGTCCAC |
Suggested primer 2: | GCATGACAGCATGAGCAGAT |