| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110204 |
| Chromosome: | chromosome 3 |
| Location: | 6137059 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191900 | (1 of 1) K11840 - ubiquitin carboxyl-terminal hydrolase 9/24 [EC:3.4.19.12] (USP9_24) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCATCCTTATCCTTGAGGCCTTGTCTCT |
| Internal bar code: | ACGCGTTGGGTGGCCTGGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 979 |
| LEAP-Seq percent confirming: | 99.5614 |
| LEAP-Seq n confirming: | 22246 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGGGTTTTAGTTGCGTA |
| Suggested primer 2: | AGAAAGGAGTGACCCGGATT |