| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110220 |
| Chromosome: | chromosome 17 |
| Location: | 1284674 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705600 | (1 of 11) PF00487 - Fatty acid desaturase (FA_desaturase) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCACCCACGCACACACACGCACAGTCG |
| Internal bar code: | ACTAGAGATTATTTATTGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 690 |
| LEAP-Seq percent confirming: | 99.7297 |
| LEAP-Seq n confirming: | 738 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCCGTACGTCATGTGCAA |
| Suggested primer 2: | TTCCTCACCCTCACCTCATC |