| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.110221 |
| Chromosome: | chromosome 9 |
| Location: | 971216 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400850 | CTL4 | (1 of 1) PTHR10877//PTHR10877:SF139 - POLYCYSTIN-RELATED // SUBFAMILY NOT NAMED; carbohydrate-binding protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCCACAGCCGCGTGGTGCAGTAGGCCC |
| Internal bar code: | TTCACTGTTCAAATTGCAACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 637 |
| LEAP-Seq percent confirming: | 99.4774 |
| LEAP-Seq n confirming: | 2094 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTACTCAGGCGAACAGGC |
| Suggested primer 2: | CAAGTAAGGTTGAGCACGCA |