| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110230 |
| Chromosome: | chromosome 17 |
| Location: | 4253847 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730550 | (1 of 3) PTHR22298:SF39 - ENDOGLUCANASE 7 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGCCACACTGCTGCGAACGAGCCGGGT |
| Internal bar code: | GGCCATGGCTGTCGGGCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 99.5157 |
| LEAP-Seq n confirming: | 411 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCCTGCGCTTGAAGTAA |
| Suggested primer 2: | TGAGTCTCAACCAAGCAACG |