| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110262 |
| Chromosome: | chromosome 11 |
| Location: | 2669082 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476650 | PUL1,PU1 | Pullulanase-type starch debranching enzyme 1; (1 of 1) 3.2.1.142//3.2.1.41 - Limit dextrinase / R-enzyme // Pullulanase / Pullulan 6-glucanohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACTACCTGCTCTCCGGCCGCTCTCTCGC |
| Internal bar code: | ACAGCCAAGGGGGTGTCTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 97.8354 |
| LEAP-Seq n confirming: | 8633 |
| LEAP-Seq n nonconfirming: | 191 |
| LEAP-Seq n unique pos: | 100 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAAGGAGCAGTCCAGAG |
| Suggested primer 2: | TGGCGACAACAAAAACACAT |