| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110268 |
| Chromosome: | chromosome 8 |
| Location: | 4373429 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381800 | (1 of 2) 5.3.3.2 - Isopentenyl-diphosphate Delta-isomerase / Methylbutenylpyrophosphate isomerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTGCTGCCAGCCAGTGTCAATGCATTG |
| Internal bar code: | GTGATCCAACCGTGTACGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 98.4702 |
| LEAP-Seq n confirming: | 3154 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTTGACCAAACCACCAGC |
| Suggested primer 2: | GTTCTGGTGCACTGTGTTGG |