| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.110282 |
| Chromosome: | chromosome 10 |
| Location: | 3923292 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448200 | ARL9 | ARF-like GTPase; (1 of 4) K07937 - ADP-ribosylation factor 1 (ARF1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTTCCTGCCGCGGAGGTGGCCCGACTC |
| Internal bar code: | AAATTCAGTGATTCTAACCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 99.0291 |
| LEAP-Seq n confirming: | 204 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGAGCCTGCGTGTAGGTA |
| Suggested primer 2: | AACACCAACTGCATGGTTCA |