| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110282 |
| Chromosome: | chromosome 11 |
| Location: | 294524 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467561 | FAP222 | Flagellar Associated Protein 222 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCGTTACGAACGTTTGCACGGCACTGCA |
| Internal bar code: | AACGAGACACCGCCTCAGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 551 |
| LEAP-Seq percent confirming: | 98.3879 |
| LEAP-Seq n confirming: | 4028 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATAGGCCTACCGTGTGCAT |
| Suggested primer 2: | CTTGCTCTTTGAGACCCCTG |