| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.110283 |
| Chromosome: | chromosome 12 |
| Location: | 8640382 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546850 | (1 of 4) PTHR11266//PTHR11266:SF42 - PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17 // SUBFAMILY NOT NAMED; Putative peroxisomal membrane protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCACGTGTAATACCTAATACAATAGGTT |
| Internal bar code: | GCACTCGTTTGTGCCCTGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 921 |
| LEAP-Seq percent confirming: | 99.6623 |
| LEAP-Seq n confirming: | 12987 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTCAACCCTAGCACGTT |
| Suggested primer 2: | AGTTACAGAGCCCCACCCTT |