| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.110300 |
| Chromosome: | chromosome 17 |
| Location: | 1706660 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708800 | GSH2 | (1 of 1) 6.3.2.3 - Glutathione synthase / GSH synthetase; Glutathione synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCGAGTAGTATGATATCTCCAGTGGCG |
| Internal bar code: | GTATGACACGAAACCGCCTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGACAGGGGACTCGCTTAG |
| Suggested primer 2: | GCTGGCAAGGCTGTAGATTC |