Insertion junction: LMJ.RY0402.110327_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.110327
Chromosome:chromosome 4
Location:2053908
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g219050 SRR10 Scavenger receptor cysteine rich (SRCR) protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATGGTGAGTGACCTCGGTTCCCCCTGCT
Internal bar code:CCGGGAAGCTAGGACGCGGAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:516
LEAP-Seq percent confirming:99.1534
LEAP-Seq n confirming:937
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTCCTCGCAATACTGAAT
Suggested primer 2:GTTACGATGGGAAAAGCGAA