| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110328 |
| Chromosome: | chromosome 17 |
| Location: | 1946071 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g710700 | (1 of 4) PTHR23257:SF490 - DUAL SPECIFICITY PROTEIN KINASE SHKE | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTCGTGCTAGCTATATTCACAGGACTA |
| Internal bar code: | TAAGATGGCGATGGAGGCGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 98.9199 |
| LEAP-Seq n confirming: | 1099 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCTTGGTCGTTTGTGTG |
| Suggested primer 2: | AACCCCTATTTGCGTCAGTG |