Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.110335 |
Chromosome: | chromosome_1 |
Location: | 2901288 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g017650 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGCCTTGTAACCAACAACAACAAGCCAAT |
Internal bar code: | CGGTTGGTCACGGACTACGAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 298 |
LEAP-Seq percent confirming: | 96.0227 |
LEAP-Seq n confirming: | 169 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAACGTGTTTTGGTCCTCT |
Suggested primer 2: | TTTCTTATGCAATCCTCCCG |