| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110343 |
| Chromosome: | chromosome 12 |
| Location: | 4334636 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520100 | CYG66 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGTCCAGCATGCGGTCGTAGCGGCTGT |
| Internal bar code: | TTGGGCAGTTTCAGGCTACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 580 |
| LEAP-Seq percent confirming: | 99.6891 |
| LEAP-Seq n confirming: | 5772 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTACATGGCTGAGGAG |
| Suggested primer 2: | TGGCGTATGAGAGTGTGGAG |