Insertion junction: LMJ.RY0402.110357_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.110357
Chromosome:chromosome 6
Location:2813063
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g271400 PGP3 (1 of 3) 3.1.3.18 - Phosphoglycolate phosphatase; Phosphoglycolate phosphatase/4-nitrophenylphosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCCAGCCCACCACCACTGCCCCGACGTC
Internal bar code:TAGGAGCGCCGTTGGCGGCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:738
LEAP-Seq percent confirming:99.3304
LEAP-Seq n confirming:2522
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:ACAGGAACAAAACCTCCGTG
Suggested primer 2:CTAGGACTGGAAGTGGCAGC