Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.110357 |
Chromosome: | chromosome 6 |
Location: | 2813063 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g271400 | PGP3 | (1 of 3) 3.1.3.18 - Phosphoglycolate phosphatase; Phosphoglycolate phosphatase/4-nitrophenylphosphatase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCAGCCCACCACCACTGCCCCGACGTC |
Internal bar code: | TAGGAGCGCCGTTGGCGGCGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 738 |
LEAP-Seq percent confirming: | 99.3304 |
LEAP-Seq n confirming: | 2522 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAGGAACAAAACCTCCGTG |
Suggested primer 2: | CTAGGACTGGAAGTGGCAGC |