| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110385 |
| Chromosome: | scaffold 24 |
| Location: | 19951 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755197 | RBD3 | Putative rubredoxin-like protein; (1 of 1) IPR004039 - Rubredoxin-type fold | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTGTAGAAAATTCTGTTGCAAGAGGCC |
| Internal bar code: | GTCACAGCTGTACTGGCGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 554 |
| LEAP-Seq percent confirming: | 99.7807 |
| LEAP-Seq n confirming: | 910 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAATTGCTGTTGAAGGA |
| Suggested primer 2: | TGCTTGTAAATGCCAGAACG |