| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110391 |
| Chromosome: | chromosome 17 |
| Location: | 5163182 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735450 | SUB14 | (1 of 14) 3.4.21.62 - Subtilisin; Subtilisin-like protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACACCCCAAAACTGTTGTCCTGCCCGAT |
| Internal bar code: | TCCTATTTTGTCAATACATCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 99.1922 |
| LEAP-Seq n confirming: | 3070 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTCTCACGCCTCACATA |
| Suggested primer 2: | AAGCAGTCGCACTGTAGGGT |