| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110395 |
| Chromosome: | chromosome 10 |
| Location: | 6433316 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465950 | CGL148 | Conserved in the Green Lineage; (1 of 1) K12846 - U4/U6.U5 tri-snRNP-associated protein 3 (SNRNP27) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCCCAAACTTTGACAGCCTGATAGACC |
| Internal bar code: | GCTGTGCCGTCGTGCACTTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 250 |
| LEAP-Seq percent confirming: | 99.2015 |
| LEAP-Seq n confirming: | 2733 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGCAGGTGTGGAGGTTA |
| Suggested primer 2: | ACGACACCATAAGTGCCCTC |