Insertion junction: LMJ.RY0402.110411_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.110411
Chromosome:chromosome 16
Location:3454178
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g670973 GSTS3,GST3 (1 of 3) 2.5.1.18//5.3.99.2 - Glutathione transferase / S-(hydroxyalkyl)glutathione lyase // Prostaglandin-D synthase / Prostaglandin-H(2) Delta-isomerase; Glutathione S-transferase, similar to Prostaglandin-D Synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCGTTGTAACTCGCTACCCACCACAAGC
Internal bar code:TATTAAGGGCGCCTATGCCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:839
LEAP-Seq percent confirming:98.6667
LEAP-Seq n confirming:74
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCTTCGAGTTCAAAGGG
Suggested primer 2:ACTCAGAAAATTGCCCATCG