| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110416 |
| Chromosome: | chromosome 3 |
| Location: | 531551 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145307 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGATGACCGCAACCCTGCCCGCAACTTC |
| Internal bar code: | GCATGGTCGCAGCCGATTCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 99.1647 |
| LEAP-Seq n confirming: | 2493 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCTTTCGACTGAACGGG |
| Suggested primer 2: | TTCTGGCAGCGTTACACAAG |