Insertion junction: LMJ.RY0402.110416_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.110416
Chromosome:chromosome 17
Location:1508202
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g707137 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTCAACCACCCAGGTATCCCGTAAAATT
Internal bar code:TCGGGATCCATTTTACGAGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:554
LEAP-Seq percent confirming:99.3151
LEAP-Seq n confirming:580
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CATCATCGCCATCAATCAAG
Suggested primer 2:CCGATTTATCGCACACACAC