| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.110445 |
| Chromosome: | chromosome 2 |
| Location: | 3579606 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095134 | (1 of 2) 2.1.1.9 - Thiol S-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGAGGCTCCCCGCGCCCTTGGTCCTGA |
| Internal bar code: | GTTTTGAAGCGTACTTGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 604 |
| LEAP-Seq percent confirming: | 97.343 |
| LEAP-Seq n confirming: | 2015 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCAGTTGCTTGGGGAAC |
| Suggested primer 2: | CTAACACAAGGCCGGGTCTA |