| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.110455 |
| Chromosome: | chromosome 10 |
| Location: | 4398479 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451450 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGCACTAGTCTTGATGTTCACGCGGAC |
| Internal bar code: | GACTGACGTTCGCACGTATCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1087 |
| LEAP-Seq percent confirming: | 99.5599 |
| LEAP-Seq n confirming: | 1131 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATAGCAATGCGCAGACGTA |
| Suggested primer 2: | GAGACACGTGCTGACGAAAA |