| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.110490 |
| Chromosome: | chromosome 2 |
| Location: | 2841336 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094650 | (1 of 1) PTHR11702:SF31 - MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTTGAGAATGGCCACCTGGTGTGAAGAG |
| Internal bar code: | CGTCGAGCGGAACGCCAGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 99.4176 |
| LEAP-Seq n confirming: | 1707 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCTGCTCTTCAACTCCT |
| Suggested primer 2: | GATGTGCCTATGACCTCCGT |