| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110499 |
| Chromosome: | chromosome 16 |
| Location: | 3663399 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688600 | XPG4 | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTTCACTGCTGCCCAAGGCGCACGGCA |
| Internal bar code: | GAGATAGTGTAGGAACGTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 85 |
| LEAP-Seq percent confirming: | 99.5169 |
| LEAP-Seq n confirming: | 1236 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTAGATACGGGAACGCAC |
| Suggested primer 2: | CTGTTAGCTGGGCTAGTCGG |