Insertion junction: LMJ.RY0402.110539_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.110539
Chromosome:chromosome_6
Location:1382812
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre06.g258800 CWP2,GP2 Hydroxyproline-rich glycoprotein component of the outer cell wal sense 5'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TTCGCGCGACGCACAGCCACCGGACCCCTT
Internal bar code:CTGCGCGGCAGCCCAATGCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:730
LEAP-Seq percent confirming:99.6534
LEAP-Seq n confirming:1725
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTGTAATCCCACCACCCA
Suggested primer 2:ATGATTTTCCGTGAGCCAAG