| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.110539 |
| Chromosome: | chromosome 6 |
| Location: | 1382812 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258800 | CWP2,GP2 | Hydroxyproline-rich glycoprotein component of the outer cell wal | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCGCGACGCACAGCCACCGGACCCCTT |
| Internal bar code: | CTGCGCGGCAGCCCAATGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 730 |
| LEAP-Seq percent confirming: | 99.6534 |
| LEAP-Seq n confirming: | 1725 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGTAATCCCACCACCCA |
| Suggested primer 2: | ATGATTTTCCGTGAGCCAAG |