Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.110556 |
Chromosome: | chromosome 12 |
Location: | 7997795 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g552001 | (1 of 4) PF01435 - Peptidase family M48 (Peptidase_M48) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAAGTGGCACTGCTGCTGCTTCCCTTTC |
Internal bar code: | TCCTTCTCAGTCAACGTGGCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 656 |
LEAP-Seq percent confirming: | 99.2941 |
LEAP-Seq n confirming: | 422 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACCTTATCCATCCCCCACT |
Suggested primer 2: | TGCGACAAGACCTCACAAAG |