| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.110597 |
| Chromosome: | chromosome 10 |
| Location: | 3713888 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446275 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTGACGCCCGAATGCGCTCCACTGGAA |
| Internal bar code: | TGGGTCACTGGATTAACGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 99.5289 |
| LEAP-Seq n confirming: | 1479 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGGAAGGCATTCAGAGG |
| Suggested primer 2: | GAGCGAGTCCCAAATCTCAG |