Insertion junction: LMJ.RY0402.110623_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.110623
Chromosome:chromosome 10
Location:2951228
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g440500 (1 of 5) PF12738 - twin BRCT domain (PTCB-BRCT) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGTCCATTCAATGCAAGCGCGTTGCACT
Internal bar code:GCGCCACAGTATGTACATCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:426
LEAP-Seq percent confirming:98.2083
LEAP-Seq n confirming:877
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCTCGACCTGACTGAAG
Suggested primer 2:CGGCAAATCAAAATGGTCTT