| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.110647 |
| Chromosome: | chromosome 6 |
| Location: | 6949115 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296200 | (1 of 4) PTHR24349//PTHR24349:SF121 - SERINE/THREONINE-PROTEIN KINASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTTCATGCGGAGCACATGGTGTCACTGT |
| Internal bar code: | CGTGTTAACCTGACGTCCTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 99.3467 |
| LEAP-Seq n confirming: | 2281 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTTGCTACTGCGCCACTG |
| Suggested primer 2: | GCGGACTGTGATTTTGGTTT |